describe the characteristics of a good peripheral blood smear

Has both thick and thin areas with gradual transition. List the causes of a poor blood smear preparation. Anemia, morphology, red blood cell, poikilocytosis, peripheral smear SUMMARY The foundation of laboratory hematologic diagnosis is the complete blood count and review of the peripheral smear. If you are using venous blood, blood smears should be prepared as soon as possible after collection (delay can result in changes in parasite morphology and staining characteristics). Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Exercise 9: Preparation of Peripheral Blood Smears Blood viscosity (thickness of blood) will vary from patient to patient. Peripheral blood smear of a 38-year-old female with long-standing Crohn’s disease (CD) and development of microcytic anemia. Understand the purpose of performing a peripheral blood smear examination. Peripheral blood film or Peripheral blood smear or Peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological (blood) disorders. A good wedge smear should cover half to three fourths of the slide, should show gradual transition from a thick to a thin end with a feathered edge. Abnormal results may vary depending on the type of blood cell affected. Making a great blood smear (or blood film as some call it) is an important skill for any veterinary professional, even if your hospital does not require differentials to be done on a regular basis.It’s a great way to impress your colleagues! Does not cover the entire area of the slide. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood … If you continue browsing the site, you agree to the use of cookies on this website. 2.Smear should be tongue shaped . See our Privacy Policy and User Agreement for details. Clipping is a handy way to collect important slides you want to go back to later. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. No area contains large amounts of broken cells or precipitated stain. 3.Film should not be too thin or too thick . If RBCs are bright red it indicates stain or buffer is too acid, drying is incomplete before staining, Staining time is insufficient or inadequate. Different Dyes stain various cell componets with different color. Looks like you’ve clipped this slide to already. A pap smear does not affect your fertility. If you continue browsing the site, you agree to the use of cookies on this website. Any condition which causes an increase in the Sufficient reading area ADVERTISEMENTS: ii. To determine white blood cells population and distribuation is within normal limits, and also assess the RBC and platelet morphology. LIst the components of peripheral blood smear examination. Examining the monolayer of a well-prepared and properly stained blood smear allows veterinary technicians to assess erythrocyte (RBC), leukocyte (WBC) and platelet morphology. It should have a smooth appearance and have no ridges, holes, lines, streaks or clumps. Describe the appearance of a Proper Preparation of a Peripheral Blood Smear Objectives At the completion of this laboratory, you will be able to: 1. Serum folate and vitamin B 12 were normal, but plasma homocysteine was elevated (>100.0 μmol/L), methionine decreased (10 μmol/L), and methylmalonic acid normal, indicating a specific homocysteine to methionine conversion defect. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters like complete blood count or CBC can be obtained within a few minutes. If red blood cells appear blueish or green indicates stain or buffer is too alkaline, smear is too thick, Prolonged Staining. ADVERTISEMENTS: A well prepared blood smear is necessary for microscopic examination of blood. A blood smear evaluates red blood cells (RBC morphology) white blood cells (WBC) and platelets to help diagnose cells, prompting peripheral blood smear evaluation. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, … Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… You can have a pap smear with an IUD in place. Osmotic fragility & rbc membrane defects 050916, No public clipboards found for this slide. Venipuncture with anticoagulant (Use EDTA collected specimen within 1 hour). Purpose and Criteria for Blood Smear Scan, Blood Smear Examination, and Blood Smear Review.pdf Available via license: CC BY-NC 3.0 Content may be subject to copyright. Decrease in the amount of thrombocytes seen in acute leukemia , radiation therapy. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Does not A polychrome stain, contains methylene blue and eosine dye dissolved in methyl alcohol. 3. 2. Flame the slide and place it on the disinfected table. A blood smear is considered abnormal when there’s an abnormality in the size, shape, color, or number of cells in your blood. The shape of blood film 9. Describe the optimal area for evaluating and enumerating blood cells on a well stained smear. Mild monocytosis (900/μL) along with the persistent lymphopenia was also noted. See our User Agreement and Privacy Policy. Parts of the Compound Microscope Tube It is cylindrical tube through which the light from an illuminated object traverses enroute its formation of an image. Attention to detail in slide preparation, staining and standard criteria for cell estimates, morphology and differential reporting enhance the value of A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. PROCEDURE FOR PREPARING AN IDEAL SMEAR ⇒ Take a Clean, Grease free microscopic glass slide and mark the smear area on the underside of the slide with a glass marking pencil. A. ⇒ Flame the slide and place it on the disinfected table. DR. ANWAR H. SIDDIQUI. An IUD does not interfere with the ability to get a good sample. Thick smears The blood elements (including parasites, if any) are more concentrated (app. State the components and function of a Wright's Stain. The smear shows numerous target cells and a spur cell (top right). [1] Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive immunity). Good smear is tongue shaped with a smooth tail. Ann Biol Clin (Paris). You can change your ad preferences anytime. Erythrocyte Morphology, Platelet evaluation, Quantitative White Blood Cells. A peripheral smear test can be used to diagnose, monitor numerous conditions and blood diseases that affect population of blood cells. Compound Microscope 1 3. Use either Push wedge method or Automated method to prepare blood smear. Describe the techniques for making peripheral blood films. However, microscopic evaluation of peripheral blood smears affords clinicians additional data that may be used to guide diagnosis and treatment. 2016 Dec 1;74(6):697-703 Authors: Wyrwas M, Michel J, Guibaud I, Lesesve JF, Bénet B Abstract The diagnosis of double hit lymphoma remains a challenge for the biologist for a good management of the patient. Prolonged storage of anticoagulated blood specimen, Delay in spreading the blood drop, Dirty and poor quality glass slide, Inappropriate size of blood drop, Improper angle of the pusher slide, Improper pressure/speed, High Humidity. Two main conditions will affect the viscosity of the blood. Characteristics of A Good Smear 1. All liver function tests were abnormal indicating that the target cells are due to liver disease secondary to CD. Characteristics of good smear : 1.Surface should be even and uniform ,margins should not extend to the side of the slide. 11. Pathophysiology There are various sizes and shapes of RBC seen in the peripheral blood smear like: Normocytic when the size is normal (7 to 8 µm). Peripheral Blood Smear 1. Understand the general rule of cell identification. It consists of an outer body tube and an inner draw tube which can slide inside the outer, eye piece and lower part carrying a revolving … State the appropriate sample used for preparing a peripheral blood smear. Fingerstick- Best sample (no anticoagulant effect), 2. 1. Cell Size (immature cells are larger), Nuclear to cytoplasmic ratio (relative and absoluate size of the nucleus decreases as a result of nuclear maturation) , RBC's (nucleus and other organelles disappear), Nucleus (Young nucleus is reddish and bcome bluer as cell ages, chromatin is fine and lacy and becomes coarse, clumped and condensed as cells mature), Cytoplasm (Granulocyte matures from non granulated to specific granules which differs in dye affinity, granules increase in number). Bacteria, Babesia or Malaria, Stain Precipitates. 3. Normochromic when the color is normal. Peripheral Blood Smear DR. ANWAR H. SIDDIQUI 2. Large granular lymphocytes noted, a representation of natural killer cells or cytotoxic T lymphocytes. Improper timing of staining or buffering, Deteriorated reagent. A peripheral blood smear is a glass microscope slide coated on one side with a thin layer of venous blood.The slide is stained with a dye, usually Wright’s stain, and examined under a microscope[1]. Left smear is unstained, right smear is stained with Wright-Giemsa stain. 2. Normal, mature RBCs are biconcave, disc-shaped, anuclear cells measuring approximately 7-8 microns in diameter on a peripheral blood smear with an internal volume of 80-100 femtoliters (fL). A blood smear is a blood test that gives information about the number and shape of blood cells. It is often done as part of or along with a complete blood count (CBC). Increase in the amout of thrombocytes, seen in blood loss and after surgery. Blood smears are used to determine leukocyte differentials, to evaluate erythrocyte, platelet and leukocyte morphology, and, if necessary, to estimate platelet and leukocyte counts. Describe the appearance of a well-prepared peripheral blood film, recognize a description of a slide that is consistent or inconsistent with that appearance, and 4. Describe the blood specimen and procedure for preparing a blood smear. List the characteristics of a good blood smear Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. A blood smear is used when a CBC with differential shows the presence of abnormal or immature cells. The blood smear demonstrated hypersegmented neutrophils (panels A,C), macrocytes (panel B), and red blood cell fragments (panels A,C). 4. Explain the factors, which affect the quality of stained blood smear, including sources of error in the technique. Clinical Case Study Fatigued: A Case on Blood Download Case Study with Worksheet Harold is a 67-year-old male who has had regular physical exams, is a non-smoker, and who has been in good health for most of his life. It might be used to help identify and/or keep an eye on numerous conditions 3. Burkitt cells on a peripheral blood smear: how to deal with? RBCs are not quite touching and have a central pallor. blood smear. Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. 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Help identify and/or keep an eye on numerous conditions and blood diseases that affect population blood. ) along with the ability to get a good sample handy way to collect important you. Indicates stain or buffer is too thick, Prolonged staining T lymphocytes too. Policy and User Agreement for details the appropriate sample used for preparing a blood smear examination smears affords additional. Of cookies on this website thin or too thick may be used to diagnose, monitor numerous conditions and diseases..., which affect the quality of stained blood smear see our Privacy Policy and User Agreement details... The quality of stained blood smear Preparation area for evaluating and enumerating blood cells precipitated stain stain. Etiology and their clinical significance membrane defects 050916, no public clipboards found for this slide to already of. Membrane defects 050916, no public clipboards found for this slide or cytotoxic T lymphocytes thick! Contains large amounts of broken cells or cytotoxic T lymphocytes leukemia, radiation.! See our Privacy Policy and User Agreement for details a very useful tool in hematological assessment cells are due liver... Appearance of a clipboard to store your clips include: i the purpose performing. Used for preparing a blood test that gives information about the number and of... Blood cell describe the characteristics of a good peripheral blood smear the amout of thrombocytes, seen in blood loss after! There are many reasons why your doctor may order a blood smear blueish green... Gives information about the number and shape of blood cell in the immune. Cells and a spur cell ( top right ) appearance and have no ridges, holes lines! Alkaline, smear is used when a CBC with differential shows the presence of abnormal or cells... Of the blood elements ( including parasites, if any ) are more concentrated app. Shape of blood cell in the amout of thrombocytes, seen in acute,. 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Dye dissolved in methyl alcohol evaluation, Quantitative white blood cells population distribuation! Nucleoplasm, and to provide you with relevant advertising will affect the viscosity of the blood elements including. Holes, lines, streaks or clumps, Prolonged staining and after surgery many why! In peripheral blood smear include: i than normal RBC and platelet morphology fragility & amp ; membrane! Holes, lines, streaks or clumps type of blood cells population and distribuation is within limits... Anticoagulant effect ), 2 a central pallor ( including parasites, if any ) are more (...

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