I recently embarrassed myself by not knowing the difference between knock-in and transgenic mice, so here’s an explanation to make sure I understand it.. In case of mouse, it was found that about 65% of the fertilized eggs survive microinjection procedure, about 25% of the implanted eggs develop into pups, and only 25% of them are transgenic. Mouse is preferred mammal for studies on gene transfers due to, a)short oestrous cycle and gestation period, 4.Technique Multiple Choice Questions and Answers on Transgenic Plant Questions and Answers Question 1 : Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of gene for ALS (acetolactate synthase) gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase) gene for GS (glutamine synthase) any of the above Answer : 1 Question 2 : In transgenic … Please take 5 seconds to Share. Thanks for visiting this site.Enjoy Biology, MCQ Biology - Learning Biology through MCQs, MCQ on Bioinformatics- Biological databases, Multiple Choice Questions on Food Preservation, Multiple Choice Questions on Sequence Alignment, Multiple Choice Questions on Gram Staining, Multiple Choice Questions on Phylum Porifera (Sponges). ***Best viewed in Google Chrome and Mozilla firefox***, 1. The difference between knock-in and transgenic mice. Inbred mice also permit studies of genetically engineered mice that are missing a gene, usually called “knockout mice,” or with an additional gene, typically called “transgenic mice.” In these examples, the gene is added or deleted in a mouse on an inbred genetic background to study the role of the genes in development and/or function. If the gene or mutation is orthologous to that in humans and causes the same disease in humans, the strain is designated as a model of the human disease. The key difference is that knock-in is targeted, meaning the desired gene is inserted into a specific locus in the target genome via homologous recombination. Hsp70 promoter of Drosophila, _________ the gene. Transgenic animals used for gene farming or molecular farming called 3. 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Hybridoma These questions can be … Post navigation David L. Vesely, in Seldin and Giebisch's The Kidney (Fifth Edition), 2013. These short objective type questions with answers are very important for Board exams as well as competitive exams. a) True b) False. ANIMAL Breeding & Transgenic Animal MCQs ANIMAL Breeding & Transgenic Animal Multiple Choice Questions :- ... DNA microinjection into the egg has been used to produce which of the following transgenic animals? First recorded attempts to maintain animal cells in culture can be attributed Answer: c Explanation: In transgenic flies, any gene linked to the hsp70 promoter is more or less inactive at room temperature, but high-level expression in all cells can be induced. The transgenic animals are those which have foreign DNA in ALL their cells. Transgenic animals used for gene farming or In plants, it is useful to restrict transgene expression to seeds. virus, Salk vaccine is inactivated form and Sabin vaccine is attenuated form of vaccines, Salk vaccines is attenuated form and Sabin vaccine is inactivated form of vaccines, a) from antigenic polysaccharide to vaccine development, b) from I see you don’t monetize proprogramming.org, don’t waste your traffic, you can earn additional bucks every month with new monetization method. In __________ it is useful to restrict transgene expression to mammary glands. Following are produced by animal cells in culture and help to adhese to culture dish except 2. These short objective type questions with answers are very important for Board exams as well as competitive exams. to, 9. (b) (i) The role of transgenic animals in vaccine safety: Transgenic mice are being used to test the safety of the polio vaccine before they are used on humans. Transgenic goats produce a variant of human tissue type plasminogen activator protein in A. blood B. urine C. milk D. muscles Ans: C. 25. The transgenic animals are created because of the benefits they provide to the man. Transgenic Animals Short Questions and Answers for competitive exams. Students can solve NCERT Class 12 Biology Biotechnology and its Applications MCQs Pdf with Answers to know their preparation level. Inducible promoters do not respond to chemicals. to a predyined antigen is given by, 5. Solve 15 MCQs on Biotechnology (Biotechnology and Its Applications) in this quiz format. All these questions were taken from the previous year question papers of CSIR JRF NET Life Sciences Examination. Drosophila heat-shock promoter is an example of ___________ a) Synthetic b) Toxic c) Naturally occurring d) Rare, 9. 8. Read more: Transgenic animals a) 1 b) 2 c) 3 d) 4, 3. Nov 13, 2012 • ericminikel. These short solved questions or quizzes are provided by Gkseries. Transgenic plants are the ones. Transgenes are genes that have been taken from one organism and transferred to the genetic makeup of another. … Mice B. Let us discuss a few of them here. For the rapid genetic progress, embryo transfer (ET) Following Multiple Choice Questions and Answers on Animal Breeding and Transgenic Animal Question 1 : Which of these established cell lines originate from a mouse embryo? After injecting the DNA, the embryo is implanted into the uterus of receptive females. Transgenic mice generated for carrying clones oncogenes and knock out mice devoid of tumour suppressing genes have rendered good prototypes for human cancer. Free PDF Download of CBSE Biology Multiple Choice Questions for Class 12 with Answers Chapter 12 Biotechnology and its Applications. a) Promoter b) Inducer c) Silencer d) Reporter, 6. Genes of some were also altered to study glucose metabolism. This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Transgenic Technology”. are produced by animal cells in culture and help to adhese to culture dish except, 2. . (a) transgenic mice for testing safety of polio vaccine before use in humans (b) transgenic models for studying new treatments for certain cardiac diseases (c) transgenic cow-Rosie which produces high fat milk for making ghee Applications Of Transgenic Animals. Biology MCQs for Class 12 Chapter Wise with Answers PDF Download was Prepared Based on Latest Exam Pattern. Thus, if one starts with 1000 fertilized eggs, only 30 to 50 transgenic pups may be produced i.e., 3-5% of the inoculated eggs develop into transgenic animals. A. These were some fields of study in which transgenic mice are involved. recombination forms, 10. a) True b) False, 8. MCQ on Animal Biotechnology 1. DNA microinjection into the egg has been used to produce which of the following transgenic animals? to produce cells capable of continuous secretion of a single type of antibody Transgenic mice are developed by injecting DNA into the oocytes or 1-2 celled embryos taken from female mice. A genetically modified mouse or genetically engineered mouse model (GEMM) is a mouse (Mus musculus) that has had its genome altered through the use of genetic engineering techniques. For example, a human gene can be copied and transferred to the genetic makeup of a mouse in order to study human disease in a model biological system. Transgenic mice lacking the gene for a dopamine re-uptake pump (Giros et al 1996) have been shown to _____ produce more dopamine; be behaviourally less active ; be very sensitive to the effects of alcohol ; be insensitive to the effects of amphetamine and cocaine ; Answer. Question 9 The expression of genes can be controlled in a spatially-controlled manner using the Cre/Lox system, and/or in a temporally controlled manner using the Tet-On/Off system. DNA insert placed within the thymine kinase gene of virus by a process of generated by introducing foreign DNA into a cell and regenerating a plant from that cell. The key difference between transgenic and knockout mice is that transgenic mice have foreign genes inserted into its genome while knockout mice have a functionally inactivated gene of interest.. Genetic engineering is the field of genetics in which the genetic makeup of an organism is modified or manipulated by recombinant DNA technology.Transgenic organisms are made by … In the mαA‐spacer‐T transgenic mice the reporter gene is dormant, since the polyadenylation element in the spacer triggers premature transcription termination, the large T protein is thus not expressed and does not cause tumor (MCQ 2: E; MCQ 3: E; MCQ 4: A; MCQ 14: B; MCQ 15: B). a) Reptiles b) Plants c) Xenopus d) Mammals, 7. A. technology, a) prepared from two different strains of polio Explanation: In transgenic flies, any gene linked to the hsp70 promoter is more or less inactive at room temperature, but high-level expression in all cells can be induced. molecular farming called, 3. species, b) from antigenic protein to vaccine development. Transgenic technology is a process by which your gene of choice is inserted into the mouse genome by random integration by either pronuclear injection of the DNA into a fertilized mouse oocyte (most common) or alternatively by electroporation into ES cells. Together with patient-derived xenografts (PDXs), GEMMs are the … Inducible transgene expression controls ______ genes. These short solved questions or quizzes are provided by Gkseries. Transgenic technology is particularly advanced in _________ a) Plants b) Animals c) Mice d) Bacteria, 2. Some genetically engineered and mutant mice strains have a mutation associated with a specific human disease. 23. Knock-in Mice vs. Transgenic Mice – What You Need to Know Transgenic and knock-in mice have existed for years. What is used to restrict transgene expression? ... Principles and Processes MCQs. Transgenic mice from retroviral vector transduction of mouse spermatogonial stem cells Analyses. a) Acidifies b) Neutralizes c) Inactivates d) Activates. MCQ Quiz No. Genetically modified mice are commonly used for research or as animal models of human diseases, and are also used for research on genes. 1. Chicken C. Pigs D. All of these Ans: D. 24. As the methods used to generate them continue to advance, more and more impressive research studies make use of these mouse models to help genetic researchers come up with new treatments and drugs. Transgenic Knockout and/or Mice Overexpressing Atrial Natriuretic Peptide Prohormone Gene. Which strain of mice do we inject? Mice B. Gene silencing involves ___________ genes. While transgenic mice, with a tendency for germline transmission were produced (Tsukui et al., 1996), similar success has not been shown in other species (Kubisch et al., 1997). The most common methods of introducing a transgene into an animal model in… a) Repressible b) Inducible c) Altered d) Toxic, 5. antigenic protein to vaccine development, d) From genome sequence to vaccine development, 8. BIOTECHNOLOGY MCQ – 07 (Advanced) (For PG Level Competitive Examinations: Set-1) Dear Students, Welcome to Biotechnology MCQ-07.This MCQ set consists of Advanced (Post Graduate Level) Biotechnology Multiple Choice Questions with Answer Key. Chicken C. Pigs D. All of these Ans: D. 24. Transgenic mice with an 11 base-pair deletion in exon 2 of the proANP gene (Figure 37.1) have increased blood pressure in homologous (−/−) mice of 8–23 mmHg compared to wild-type (+/+) mice.